Rotavirus VP6 Expressed by PVX Vectors in Nicotiana benthamiana Coats PVX Rods and Also Assembles into Viruslike Particles
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چکیده
منابع مشابه
Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana
Agroinfiltration and PVX agroinfection are two efficient transient expression assays for functional analysis of candidate genes in plants. The most commonly used agent for agroinfiltration is Agrobacterium tumefaciens, a pathogen of many dicot plant species. This implies that agroinfiltration can be applied to many plant species. Here, we present our protocols and expected results when applying...
متن کاملProduction of Hybrid Chimeric PVX Particles Using a Combination of TMV and PVX-Based Expression Vectors
We have generated hybrid chimeric potato virus X (PVX) particles by coexpression of different PVX coat protein fusions utilizing tobacco mosaic virus (TMV) and PVX-based expression vectors. Coinfection was achieved with a modified PVX overcoat vector displaying a fluorescent protein and a TMV vector expressing another PVX fluorescent overcoat fusion protein. Coexpression of the PVX-CP fusions i...
متن کاملPVX-potyvirus synergistic infections differentially alter microRNA accumulation in Nicotiana benthamiana.
In comparison to single infections, co-infection of Nicotiana benthamiana with Potato virus X (PVX) and Potato virus Y (PVY) or Plum pox virus (PPV), resulted in increased systemic symptoms (synergism in pathology). Previous studies have shown that virus infections affected the accumulation of various microRNAs (miRNAs) and miRNA target genes. Our studies revealed that double infection by PVX a...
متن کاملTransient expression of the cancer/testis cancer antigen NY-ESO-1 in Nicotiana benthamiana using a PVX-based viral vector
Background Cancer/testis antigens is a group of proteins which expression is usually restricted to the human germ line but is also present at high levels in several types of cancer cells [1]. The antigen NY-ESO-1 is one of these antigens showing high immunogenicity and, therefore, holding great potential for a cancer vaccine [2]. Its heterologous expression is being tested in different expressi...
متن کاملRecombinant rabbit hemorrhagic disease virus capsid protein expressed in baculovirus self-assembles into viruslike particles and induces protection.
VP60, the unique component of rabbit hemorrhagic disease virus capsid, was expressed in the baculovirus system. The recombinant VP60, released in the supernatant of infected insect cells, assembled without the need of any other viral component to form viruslike particles (VLPs), structurally and immunologically indistinguishable from the rabbit hemorrhagic disease virion. Intramuscular vaccinat...
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ژورنال
عنوان ژورنال: Virology
سال: 2000
ISSN: 0042-6822
DOI: 10.1006/viro.2000.0314